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Fig. 4. Confocal and whole-mount immunohistochemical analyses of GATA4 (red) and PECAM1 (green) expression in B6 E13.5 gonad-mesonephros complexes: Dax1-/+ (A), Dax1-/YAKR (B), Dax1+/YAKR (C) and Dax1-/YAKR Tg2 (D). Dax1+/YAKR and Dax1-/YAKR Tg2 gonads have normal testis morphology and upregulated GATA4 expression in Sertoli cells compared with Dax1-/+ and Dax1-/YAKR gonads. PECAM expression is visible on the surface of germ cells and vascular endothelial cells. In each image, the gonad is at the top and the mesonephros at the bottom.





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