spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.



Fig. 3. Expression of Sgk is dependent on Hoxd gene function in genitals and forelimb buds. (A) Sgk expression appears distally in E11 wild-type limb buds. At E12.5 and E13.5, expression is prominent in the proximal portion of the interdigital zone. By contrast, Sgk expression in mutant HoxDDel1-13 limbs is largely limited to a proximal domain at E12.5, with little interdigital staining. (B) In the developing genital bud, Sgk is expressed in a distal domain of the ventral mesenchyme, adjacent to the urethral epithelium (arrow). This domain is not visible in mutant genital buds. (C) Real-time RT-PCR measurements mirror the WISH results, with highly significant (**P<0.01) differential expression in both limbs and genitals. Although the differential expression was initially detected in genitals, the highest fold change in the developmental series was measured in limbs (2.36-fold at E11.5).





Right arrow Return to article