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Fig. 1. Apical capping of anterior polar cells with basement membrane material. (A)
Formation and migration of the BC cluster. (a) A stage 8 egg chamber made of a
monolayer epithelium (follicle cells, light gray) surrounding the germline
(blue, nurse cells; yellow, oocyte). (b) Anterior polar cells (orange) express
the Upd ligand activating the JAK/STAT pathway in follicle cells (arrows). (c)
Cells receiving the highest levels of Upd are committed into the outer BC fate
(beige). (d) A mature cluster delaminates and starts migration. (B) GFP-Vkg
(green) accumulates in the BM. At stage 8, an apical cap forms over anterior
polar cells (arrowhead). (C,D) Close up of the framed regions shown in B.
Polar cells are marked by Fas2 expression (B-D; red). (E-G) Co-localization of
GFP-Vkg and Coll.IV 
1 (F,G; red), at the BM and in the apical cap (G).
(H) Antibodies are specific to Coll.IV 1, as they do not recognize
GFP-Vkg. The lower band (asterisk) is non-specific. w, white. (I-K)
Co-localization of GFP-Vkg and Laminin A (red) in the apical cap. (L-N) Apical
cap accumulation of BM protein-trap lines G205 (Vkg), 2840 and 2867 (Perlecan;
see Fig. S1 in the supplementary material). Anterior is to the left. Scale
bars: in B, 10 µM; in C, 5 µM for C,D; in E, 5 µM for E-G; in I, 5
µM for I-K; in L, 5 µM for L-M.
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