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Fig. 3. The apical cap is asymmetric and dynamic. (A-F) Three-dimensional imaging
and reconstruction of the anterior region of stage 8 egg chambers, showing
GFP-Vkg (green), Fas2 (red) and Hoeschst (blue, in A). (A) The original stack
is a projection of 20 z sections (total depth is 7.6 µm). (B)
Intermediate processing showing BM (green), polar cells (white) and apical cap
(blue). (C) The resulting 3D-reconstructed polar cells with the apical cap.
(D-F) Three other examples of reconstructed polar cells with their apical cap.
Insets in C-F show the apical side of polar cells. The red line is the
boundary between the two polar cells. (G-O) GFP-Vkg (green; G-0) egg chambers
showing Fas2 (G-L), Crumbs (M,O) or Fas3 (N) in red. The process of apical
capping is transient and can be described in four discrete phases (1-4; see
text for details). After shedding (L), the BC delaminate and invade the nurse
cell compartment (M). During migration (phase 5), a BM containing GFP-Vkg is
present in polar cells specifically (M-O), opposite to the apical side (marked
with Crumbs in M and O). The polar cell BM is maintained when BCs reach the
oocyte (O). Anterior is to the left. Scale bars: in A, 5 µM for A-F; in G,
5 µm for G-O.
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