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Fig. 2. Larval muscle arm outgrowth and migration. (A) Dorsal view of eight BWMs of
an early RP127 trIs25 L1. Muscles 9-15 of the dorsal right quadrant
(blue arrows) each BWM extends a single arm (red arrow) to the dorsal cord
(yellow arrow). (B) Dorsal view of the same eight BWMs as in A, but in a
different animal of the early L2 stage. The four muscles of the dorsal right
quadrant (blue arrows) extend 3-4 arms to the dorsal cord. In both A and B,
anterior is to the left. (C) Four dorsal left BWMs of an adult RP242
Ex[him-4p::MB::YFP; hmr-1b:: DsRed2; unc-129nsp:: DsRed2]; dpy-5(e61)
animal, which serves as the control for muscle arm counts in a lin-6
mutant background. BWM identity numbers are shown, along with the number of
muscle arms in brackets. (D) The same four BWMs as in C, but in an RP226
Ex[him-4p::MB::YFP; hmr-1b:: DsRed2; unc-129nsp:: DsRed2]; lin-6(e1466)
dpy-5(e61) animal. (E-P) RP235 animals mosaic for [myo-3p::Mb::YFP;
unc-25p::DsRed; unc-129nsp::CFP]. Muscle arms are indicated with a red
arrowhead. (E-H) The same ventral left muscle 13 from four different animals.
(I-L) The same dorsal right muscle 13 from four different animals. (M-P) The
same ventral right muscle 13 from four different animals. Scale bars: 20
µm.
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