DEV01889 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure 1 (Adobe PDF) -

  Fig. S1. Requirement of lin for A8-specific expression of wg and rho. rho (A,B) and wg (C,D) transcripts in mid-stage 11 wild-type (A,C) or lin^G75 mutant (B,D) embryos. Dotted circles indicate the A8-specific expression of wg and rho, which is lost in lin mutants.

• Supplemental Figure 2 (Adobe PDF) -

  Fig. S2. Independent requirements of Wg and Egfr signalling for posterior spiracle organogenesis. (A,B) Wg and Egfr signalling are not required for the establishment of A8-specific expression of rho and wg, respectively. (A) Egfr^f2 embryos stained for wg transcripts. (B) wg^CX4 embryos stained for rho transcripts. (C,D) Loss of Egfr signalling in spiracular chamber or stigmatophore cells can be rescued by expressing RasV12 in the same cells. ems-Gal4/UAS-DN-Egfr/UAS-rasv12 (C) and sal-Gal4/UAS-DN-Egfr/UAS-rasv12 (D) embryos display differentiated filzkörpers (arrows, compare with Fig. 4F) and stigmatophores (white bracket, compare with Fig. 4I). (E-H) Wg and Egfr signalling can not substitute each other. Loss of Wg signalling in spiracular chamber (E; ems-Gal4/UAS-DN-TCF/UAS-spi^S) or stigmatophore (G; sal-Gal4/UAS-DN-TCF/UAS-spi^S) cells cannot be rescued by providing Egfr signalling in the same cells. Similarly, loss of Egfr signalling in spiracular chamber (F; ems-Gal4/UAS-DN-Egfr/UAS-wg) or stigmatophore (H; sal-Gal4/UAS-DN-Egfr/UAS-wg) cells can not be rescued by providing Wg signalling in the same cells.

• Supplemental Figure 3 (Adobe PDF) -

  Fig. S3. Precursors of anterior spiracular hairs are En positive at stage 13. Mirr (A,C) and En/Mir (B,D) labeling in stage 12 (A,B) and 13 (C,D) wild-type embryos. The spiracular hair sensory organ precursors can be detected as four clusters of Mirr-positive cells (circles). At stage 12, two clusters are located in the anterior compartment (B), while at stage 13, all four clusters are located within the En-positive region (D).