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Fig. 4. Wg, Hh and Egfr signalling are required in spiracular chamber and
stigmatophore cells for posterior spiracle organogenesis. (A-D)
ems-Gal4 or sal-Gal4 promote expression at late stage 11.
ems-Gal4/UAS-GFP (A,B) and sal-Gal4/UAS-GFP embryos (C,D),
immunostained in red for GFP, and in green for Cut (A,B) and Sal (C,D) at
early (A,C) or late (B,D) stage 11. GFP is detected in late stage 11 embryos,
roughly 1 hour after the onset of Sal and Cut protein accumulation in the
dorsal A8 ectoderm.(E-J) Effects of DN-TCF (E,H), DN-Egfr (F,I) and DN-Ci
(G,J) expression driven by ems-Gal4 or sal-Gal4. Blocking
either signalling by ems-Gal4 impairs filzkörper formation, and
not that of stigmatophore (E-G). Reciprocally, blocking either signalling by
sal-Gal4 impairs stigmatophore development, leaving filzkörper
differentiation unaffected (H-J). The stigmatophore phenotype resulting from
the loss of Hh signalling (J) is weaker than those resulting from the loss of
Wg and Egfr signalling (H,I).
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