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Fig. 6. AbdB and Hh signalling control A8-specific patterns of wg and rho. (A-C) In situ hybridisation (green) to rho (A), wg (B) and hh (C) transcripts in mid-stage 11 AbdB mutant embryos. rho and wg fail to adopt A8-specific patterns (circles; compare A with Fig. 1P and B with Fig. 1J). (D,E) 69B-Gal4/UAS-AbdB embryos stained in red for Cut protein and in green for rho (D) or wg (E) RNA. The expression of wg and rho in all trunk segments mimics expression found in wild-type A8. However, wg is expressed in more than three cells (compare with Fig. 1M). (F,G). In situ hybridisation (green) to rho (F) or wg (G) transcripts in mid-stage 11 hh^IJ35 mutant embryos. As in AbdB mutant embryos, rho and wg patterns do not acquire A8-specific properties (circles). (H-K) Magnified views of ems-lacZ embryos stained for ß-Gal (red) and wg (H,I) or rho (J,K) transcripts (green). ß-Gal is detected before (H) and after (I) wg has adopted an A8-specific pattern. The transition of the rho expression pattern occurs slightly later than that of wg, as ß-gal is not detected prior to rho expression (J), but concomitantly with its expression in the posterior dorsal cell cluster (K).

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