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Fig. 2. Perturbed expression of Hox11 and Wt1, known regulators
of spleen development, in Pbx1-/- embryos. Transcripts for
the spleen regulators Hox11, Wt1, Pod1 and Nkx3.2 were
detected by in situ hybridization to frozen sagittal sections of wild-type and
Pbx1-/- upper abdominal organs at E11-11.5 and E12-12.5,
as indicated. The splenic primordium is outlined by black dashes. (A-D) Both
at E11-11.5 and E12-12.5 Hox11 expression is absent in the spleen
primordia of Pbx1-/- embryos, compared with wild-type
littermates. (E-H) Wt1 expression is also absent in spleen anlage of
Pbx1-/- embryos compared with wild-type littermates both
at E11-11.5 and E12-12.5. It is noteworthy that Wt1 expression is
still present in the mesothelial lining that surrounds the mesenchyme of the
splenic anlage (arrow). (I-L) Pod1 and (M-P) Nkx3.2 are
normally expressed within the developing spleen primordia of
Pbx1-/- embryos compared with wild-type littermates, both
at E11-11.5 and E12-12.5. L, liver; Sp, spleen; St, stomach.
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