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Fig. 8. Transplants suggest that tfap2a and tfap2b are required
in the pharyngeal ectoderm, and act non-autonomously in NCCs. Each column
represents photos taken at different stages of the same animal. Columns 1 and
2 show transplants from wild-type donors into low mutant hosts,
whereas the host in column 3 is a low mutant injected with ap2bMO.
Prospective cranial ectodermal cells were transplanted from donor embryos
labeled with a lineage tracer into unlabeled hosts at 4-5 hpf (A). Lateral
views of living mosaic embryos at 28 hpf (A-G) showing that transplanted cells
(red rhodamine-labeled cells in E-G) form patches of ectoderm covering parts
of the face and arches. (H,I) Ventral views of mosaic larvae at 4 dpf stained
for biotinylated lineage tracer (brown) in the ectoderm. Arrows in H indicate
transplanted donor cells in ectoderm. Arrowheads in H,I indicate rescued
cartilage of the ceratohyal. (J) fli1-GFP expression in the same embryo as G.
(K,M) Flat-mounted rescued cartilages (blue) with ectoderm attached (brown).
(L) Higher magnification view of the larvae shown in I, in which transplanted
ectodermal cells and cartilage can be clearly distinguished. (O) Section
through rescued ceratohyal cartilage in K. (N,P) Diagrams of cartilages shown
in K and M. cb, ceratobranchial; ch, ceratohyal; e, ethmoid; h, hyoid arch;
hs, hyosymplectic; m, mandibular arch; mc, Meckel's cartilage; ot, otic
vesicle; pc, parachordals; pq, palatoquadrate; se, surface ectoderm; t,
trabeculae. Scale bars: 50 µm.
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