NKX6 transcription factor activity is required for - and ß-cell development in the pancreas

DEV01875 Supplementary Material

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• Supplemental Figure 1 (Adobe PDF) -

  Fig. S1. The anti-NKX6.2 antibody specifically detects NKX6.2. Immunofluorescence detection of NKX6.2 on transverse sections from wild-type Nkx6.2^–/– embryos at E11.5. Although NKX6.2 marks the cells of the stomach epithelium (s) in wild type (A), no NKX6.2 protein is detected in Nkx6.2^–/– embryos (B). The area outlined in B indicates the stomach epithelium. (C) PDX1 marks the cells of the stomach epithelium on an adjacent section to (B). Scale bar: 50 mm.

• Supplemental Figure 2 (Adobe PDF) -

  Fig. S2. NKX6 activity is not required for the expression of Pax4 or HB9. In situ hybridization for Pax4 (A-D) and immunofluorescence detection of HB9 with insulin (E-H) in pancreas from wild-type, Nkx6.2^–/–, Nkx6.1^–/– and Nkx6.1^–/–;Nkx6.2^–/– embryos at E14.5. Pax4 (A-D) and HB9 (E-H) expression is similar in wild type and all Nkx6 compound mutants. (I) Quantification of HB9⁺ cells in wild type and Nkx6 compound mutants at E14.5 and E18.5. The number of HB9⁺ cells per section was determined by counting immunofluorescence-labeled cells. Values are shown as average numbers (wild type arbitrarily set to 100%)ąs.e.m. from four independent pancreata per genotype. Scale bar: 50 mm.