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Fig. 9. Heatshock-induced ectopic expression of lmx1b.1, wnt1 and fgf8. (B,D,F) One-cell stage embryos were injected with Hsp70-Lmx1b.1 (B,D) or Hsp70-Lmx1b.2 (F), heat shocked during early somitogenesis, and fixed approximately 12 hours after heat shock. Embryos were then examined for lmx1b.1, wnt1 or fgf8 expression by whole-mount in situ hybridization. (A,C,E) Uninjected, heat-shocked embryos were used as controls. Lateral views with anterior leftwards and dorsal upwards are shown. Red arrowheads indicate position of MMR and white arrows indicate ectopic clones.





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