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Fig. 3. Cloning of the sma-5 gene, mRNA structure and RNAi experiments.
(A) Physical map of the region around sma-5 gene. The YAC DNA clone,
cosmid DNA clones and PCR fragments used for identification of the
sma-5 gene are shown. Thick lines represent regions carrying
functional sma-5 gene. (B) Location of deletions in W06B3.2 and
structure of mRNAs. White and grey rectangles indicate coding and non-coding
exons, respectively. (C) RNAi experiments. Average volumes of progeny born
after injection of dsRNA of W06B3.2 into N2 (RNAi-1, 2, 3), control progeny
born from a parent injected with water or sma-5(n678) mutant worms
are shown together with standard deviations. Two-day-old adult animals were
used for measurement.
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