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Fig. 7. Effect of expression of extrachromosomal sma-5 genomic gene
(second group of columns) or sma-5 cDNA under the indicated promoter
(lower groups of columns) in the sma-5 (n678) mutant on body and
organ volume in 2-day-old adults. Body volumes relative to those of the
wild-type body volume (white bar), and intestinal volumes (gray bar) or
hypodermal volumes (black bar) relative to those of the dss-1::gfp or
col-19p::gfp transgenic lines in the wild-type background are shown
by bars. The top group of columns show the results of the sma-5
(n678) mutant. T-shaped bars represent s.e.m., which was calculated by
the formula
(fDx2/y2+x2fDy2/y4)1/2/n1/2
where x and fDx are mean and s.d. of the volume of the indicated strain, and y
and fDy are those of the wild-type volume
(Bevington and Robinson, 2003).
The smaller of the numbers of worms examined for the indicated and the
wild-type worms was taken as n. An asterisk indicates significant
difference in t-tests (P
0.05) from the value for the
sma-5 mutant. One-hundred percent (wild-type volume) corresponds to
4.39±0.41 nl, 0.88±0.068 nl and 1.01±0.20 nl for body,
intestinal and hypodermal volumes, respectively. Hypodermal volume of a worm
expressing sma-5 cDNA under dss-1 promoter was not obtained
because a corresponding transgenic line was not obtained.
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