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Fig. 3. FRK-1 is required for late epidermal differentiation. (A) Percent of
embryos with a wild-type (empty bars) and frk-1(RNAi) (filled bars)
genotype expressing the indicated marker. Numbers of frk-1(RNAi)
embryos scored are shown above each bar. More than 100 embryos were scored for
all markers in wild-type embryos. Seam cell marker (SCM) is a transgenic
reporter that expresses a GFP-ß-galactosidase fusion protein specifically
in seam cells driven by an uncharacterized promoter
(Terns et al., 1997). (B)
Nomarski images of mutant and rescued embryos. Expression of the
frk-1 coding region from a pan-epidermal promoter, elt-1p,
restores enclosure and elongation to homozygous mDf7 embryos.
Expression in non-seam epidermis only, using the elt-3 promoter, is
not sufficient to allow enclosure or elongation. (C) Genetic model for the
pathway of epidermal differentiation, showing position of FRK-1 action based
on gene expression data.
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