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Fig. 5. Scanning electron micrographs of E15 (A-H) and E16 (K, L) eyelids from
Fgf10+/– (A,C,E,G,K) and
Fgf10–/– (B,D,F,H,L) fetuses. C,E,G and D,F,H
are higher magnifications of A and B, respectively. (C,D) The inner canthus
region, (E,F) the lower eyelid margin, and (G,H) the outer canthus region.
Clumps of rounded periderm cells are observed in the inner canthus of the
Fgf10–/– mutant (D), but rarely seen in the
outer canthus (H). (E) The arrowheads indicate a regular accumulation of
epithelial cells, with the future periderm cells lined up on the eyelid margin
of the heterozygote. (F) In the homozygote, rounded periderm cells are
scattered away from the eyelid margin. (I,J) Transmission electron micrographs
of the eyelid tip epithelium from wild-type (I) and
Fgf10–/– (J) E15 fetuses. Section near the
outer canthus region. Insets show higher magnifications of filopodia of a
leading edge cell (indicated by an arrowhead). (I) Numerous filopodia are
produced. (J) In the Fgf10–/– mutant leading
edge, epidermal cells still produce filopodia, although these are distinctly
fewer and shorter. co, cornea. (K,L) Fgf10+/–eyelids
are fused, whereas Fgf10-null ones are wide open. Scale bars: 380
µm (A,B); 60 µm (C-H); 5 µm (I,J); 300 µm (K); 500 µm (L).
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