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Fig. 6. Cultures and analyses of primary keratinocytes. (A) In vitro scratch assay.
Both the wild-type and mutant keratinocytes migrated into the gap within 20
hours. (B-E) Laser scanning microscopic analysis of actin organization in
wild-type and mutant keratinocytes at the leading edge of a scratch assay
performed as above 5 hours post-scratch. The cells were fixed and stained with
phalloidin. Lamellipodia (B,C) and filopodia (D,E) are observed in both the
wild-type and mutant keratinocytes, although in the mutant the lamellipodia
are often not oriented toward the gap (arrows in C; arrowheads in B for
comparison) and the filopodia appear thinner. Scale bars: 100 µm (A; all
images are the same magnification); 50 µm (B,C); 10 µm (D,E).
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