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Fig. 8. Expression of Shh, activin ßB, and Tgfa during
eyelid closure of normal and Fgf10-null mice. To better visualize the
normal expression pattern, a mouse strain with no pigment in the eye was used,
as shown in A-C,L,M,R,S. Whole-mount in situ hybridization of the eyes (A-O,
R-U) and section in situ hybridization of the upper eyelid primordia
(P,Q,V,W). Higher magnifications of the upper (H,I,N,O,T,U) and lower (J,K)
eyelid margins. In A-O, R-U, the inner canthus is to the right, with the outer
canthus to the left. (A-C) Shh is expressed along the upper eyelid
margin and the temporal canthus at E13.5. By E15, Shh expression is
also detected along the lower eyelid margin and in the prospective upper
eyelash. Shortly after that, Shh expression along the eyelid margin
becomes down-regulated and restricted to the eyelash anlagen. (D-G) The onset
of Shh expression along the eyelid margin is delayed in
Fgf10–/– mutants. The eyes shown in D,E and
F,G were from the same littermates, respectively, and were processed for in
situ hybridization simultaneously. In Fgf10-null eyelids,
Shh expression appears down-regulated at E13.5 (E) and up-regulated
at E15 (G,I,K), as compared with normal eyelids (D,F,H,J). (L,P) At E15,
activin ßB is expressed by the leading edge cells (arrow in P) on the
eyelid margin, the future periderm cells. (M) By E16, activin ßB is
expressed by the periderm cells at the fusion line. (N-Q) The expression of
activin ßB is down-regulated on the Fgf10-null eyelid margin, as
compared with normal littermates. (R,V) Tgfa mRNA is concentrated in
the leading edge cells (arrow in V) on the eyelid margin, the future periderm
cells. (S) By E16, Tgfa is expressed by the periderm cells of the
fusing eyelids. (T-W) The expression of Tgfa is down-regulated or
more diffuse at the Fgf10-null eyelid margin, as compared with normal
littermates. Scale bars: 50 µm (P,Q,V,W).
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