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Fig. 9. FGF10 protein induces up-regulation of activin ßB and Tgfa
mRNAs and promotes eyelid closure in an explant culture. An FGF10- or
PBS-soaked bead was implanted in the eyelid mesenchyme. The expression
patterns of activin ßB, Tgfa and F-actin were examined 12 hours
after the culture of E15 normal eyelid anlagen. (A-D) The expression domain of
activin ßB (indicated by the dotted lines in A and B) was enlarged after
FGF10 bead implantation (B). (C) Higher magnification of the area boxed in B.
Activin ßB is expressed by subsets of cells in the thickened epidermis
after FGF10 application. (D) Histology of a serial section of the explant
shown in C and I. The epidermis (shown by the green line) in the vicinity of
an FGF10 bead was thickened, as compared with that in F. (E) Activin ßB
is not expressed in the normal epidermis abutting a PBS bead. (F) Histology of
a serial section of the explant shown in E and J. Normal thickness of the
epidermis is indicated by the green line. (G-J) The expression domain of
Tgfa is enlarged after FGF10-bead implantation (H). The arrowhead in
G and H indicates the leading edge of the eyelid. (I) Tgfa is
distinctly expressed in the thickened epithelium abutting the FGF10-bead.
(K,L) Phalloidin staining of the eyelid tips after FGF10-bead (K) or PBS-bead
(L) implantation. The area showing accumulation of F-actin is enlarged after
FGF10 application (arrowheads in K). The arrows in K and L indicate the
epithelial leading edge. (M) Schematic representation of an explant culture of
Fgf10-null eyelid anlagen and calculation of the percentage of eyelid
closure after FGF10 application. The area of the eyelid opening was measured
before and after the culture. Two beads were implanted in the eyelid
mesenchyme. (N) FGF10 protein can promote eyelid closure in the
Fgf10-null eyelid anlagen. ActB, activin ßB; bd, bead; co,
cornea; lld, lower eyelid; uld, upper eyelid. Scale bars: 100 µm (A,B,G,H);
50 µm (C-F,I,J); 50 µm (K,L).
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