|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 3. Immunoneutralization of candidate anti-proliferative and differentiating
factors in CMCerebellum and replication of effects by exogenous
BDNF and TGFß2. (A) CMCerebellum was preadsorbed with the
indicated dilutions of anti-NGF, BDNF or TGFß2. (B) Immunoneutralization
of BDNF and TGFß2 significantly attenuates the anti-proliferative actions
of CMCerebellum; anti-TGFß2 significantly attenuates the
pro-differentiating effects of CMCerebellum, assessed by
MAP2A/MAP2B expression. (C,D) Exogenous BDNF (C) and TGFß2 (D)
dose-dependently inhibit BrdU retention in hippocampal cells; the inset (C),
shows that transient expression of pBDNF also reduces BrdU incorporation
(pEGFP used as transfection control). (E) Exogenous BDNF and TGFß2
promote neuronal maturation in hippocampal cultures (increased expression of
MAP2A/MAP2B and neurons with neurite lengths more than twice the diameter of
the soma). The control data in E, shown as 100%, represent 126/865
MAP2-positive cells (14.5%) with `long neurites' in the BDNF studies, and
94/518 MAP2-positive cells (18.2%) with `long neurites' in the TGFß2
experiments. Numerical data refer to mean±s.d. (n=4-6)
*P<0.05, **P<0.01, ***P<0.001 (versus
appropriate controls).
|
