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Fig. 1. Shh, Pax6, Pax7 and Msx1 are expressed in the differentiated and regenerating axolotl spinal cord. All panels are cross-sections with the dorsal side up. (A-C) Shh is expressed in the floor plate of the differentiated spinal cord (A) and in the ventralmost ependymal cells 6 dpa (B,C). The overview in C shows that Shh is expressed exclusively in the spinal cord. (D,E) Pax6 is expressed in the lateral cells of the differentiated axolotl spinal cord (D) and in the lateral ependymal cells 8 dpa (E). (F,G) Pax7 is expressed in the dorsolateral cells of the differentiated spinal cord (F) and in the dorsolateral domain of the ependymal tube 6 dpa. In addition, Pax7 is expressed in lateral tail cells (F,G). (H-J) Msx1 is expressed in the roof plate of the differentiated spinal cord (H) and in the dorsalmost ependymal cells 4 and 5 dpa (I,J for overview). Panels A-E,H-J show in situ hybridizations, panels F,G antibody staining. Scale bars: 100 µm in A,B,E-G; 50 µm in C,D,H-J.





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