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Fig. 3. Dorsal spinal cord progenitor domains are increased in cyclopamine-treated
and normal in rescued regenerates. (A-C) Pax7 antibody stainings on
cross-sections of cyclopamine-treated regenerate (A), rescued regenerate (B),
and agonist-treated regenerate (C). The Pax7 expression domain in the dorsal
ependymal tube is expanded ventrally in A, normal in B (compare to
Fig. 1G), and absent in C
(dashed line in C marks the ependymal tube). The arrows in A,B point to the
ventral border of Pax7 expression in the ependymal tube. Note that the Pax7
expression persists in the lateral blastema cells in C. Pax7 staining is in
red and nuclear Hoechst staining in blue. (D,E) In situ hybridization of
Msx1 on cross-sections of cyclopamine-treated (D) and rescued
regenerates (E). Msx1 expression in the ependymal tube of
cyclopamine-treated regenerates is strongly expanded laterally (D, compare
with Fig. 1I). The rescued
tails show normal Msx1 expression (F). Scale bars: 100 µm.
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