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Fig. 5. Sox9 and Pax7 expression is reduced in cyclopamine-treated
blastemas. (A-C) Sox9 in situ hybridization on cross-sections of
control (A), cyclopamine-treated (B) and agonist-treated (C) regenerates 6
dpa. Note the absence of staining in B. The arrows in C point to
Sox9-positive dorsal blastema cells. (D) Quantification of
Pax7-positive blastema cells in control and cyclopamine-treated regenerates 6
dpa. Columns represent the mean percentage of Pax7-positive blastema cells of
three regenerates (total number of counted cells per regenerate is between 704
and 1128). Error bars indicate standard deviations. The t-test value
is 0.0007. Scale bar: 100 µm in A-C.
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