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Fig. 1. Primary neurogenesis requires Gli1, Gli2 and Gli3. (A) PAGE analyses of in
vitro transcription/translation of Gli1, Gli2 and Gli3 cDNAs
mixed with 1 µM of each MO. (B) Percentage of reduction of
N-tubulin+ primary neurons: motoneurons (M), interneurons
(I), sensory neurons (S) and total neurons (T) in stage 14 embryos injected
with each MO. Neurons were counted in the embryos with reduced
N-tubulin expression. The percentage of each cell type was calculated
by dividing the number of neurons on the injected side by the number of
neurons on the control side. The statistical analysis was performed using
Student's t-test. Error bars are s.e.m. (C-H) Examples of
N-tubulin expression on stage 14 embryos injected (inj.) with MO-1
(C), MO-2 (D), MO-3 (E,G), MO-C (F) and MO-3 plus GLI3 RNA (H), the latter
showing phenotypic rescue. The ß-gal tracer is red.
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