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Fig. 4. Gli-induced neurogenesis requires endogenous Gli function. (A,C) N-tubulin expression on stage 14 embryos co-injected (inj.) with Gli1 or human GLI1 and each MO. Arrows indicate ectopic neuronal differentiation. The ß-gal tracer is red. (B,D) Percentage change of primary neurons: motoneurons (M), interneurons (I), sensory neurons (S) and total neurons (T) in stage 14 embryos injected with Gli1 (B) or human GLI1 (D) and each MO. Neurons were counted in the embryos that showed a phenotype. The percentage of each cell type was calculated by dividing the number of neurons on the injected side by the number of neurons on the control side. The statistical analysis was performed using Student's t-test. Error bars are s.e.m.





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