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Fig. 3. hGfap-cre+/–;Ptenloxp/loxp mice show
defects in granule neuron migration in vivo. (A) Granule cell migration was
analyzed by BrdU pulse labeling. Mice were injected 100 mg/kg BrdU
peritoneally and distribution of BrdU-labeled neurons was determined at 20
hours and 110 hours after injection. At 110 hours post-BrdU labeling,
increased number of granule cells was seen in the ML of PTEN mutant mice (A,
b') compared with littermate controls (A, a'). A histogram
(quantitation of BrdU-labeled neurons within the ML) indicates that
Pten mutant mice had a significant increase in cell number at 110
hours post-BrdU injection (
P<0.001). (B) Ki67 and
phospho-histone H3 immunostaining indicated that the proliferation zone in the
EGL was largely maintained with no apparent ectopic proliferation within the
ML. EGL, external granule layer; ML, molecular layer; PL, Purkinje cell layer;
IGL, internal granule layer; WM, white matter. Scale bar: 50 µm.
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