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Fig. 3. The vascular sinusoidal network is disorganized in mutant livers. (A,C,E,G) Control livers; (B,D,F,H) mutant livers. (A-D) H&E staining of unperfused (A,B) and perfused (C,D) newborn livers shows that red blood cells accumulate in the livers of TRE-VEGFR2T/LAP-tTA transgenic newborns and cannot be flushed out (D). (E,F) Anti-CD31 staining (brown) shows that the microvascular networks in TRE-VEGFR2T/LAP-tTA livers fail to fully develop when compared with controls, with some sinusoids appearing to be collapsed (arrow in F). (G,H) Anti-LYVE1 staining (brown) shows a similar expression pattern and level.





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