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Fig. 2. Generation of Carp-Lbh transgenic mice. (A) Schematic of the
Carp-Lbh transgene. A 2.5 kb promoter of Carp drives
cardiomyocyte-specific expression of a Flag-tagged Lbh transgene.
Other elements of the transgenic construct are a ß-globin
intervening region (IVS), a 3' untranslated region (UTR) consisting of
part of the lacZ gene and a SV40 polyA (pA) site. P1 and P2, probes
used for RNA in situ hybridization shown in C-H. (B) A 6-month-old transgenic
(Tg) and a wild-type (wt) littermate. Note hunched body posture and lethargy
of the Tg animal. (C-H) Comparison of endogenous Lbh expression in wt
(C,E,G) with expression of the Lbh transgene in Tg embryos (D,F,H) at
embryonic day 9.5 (E9.5) and E12.5 by RNA in situ hybridization using probes
P1 or P2 as indicated. Right and left views of E9.5 whole mount embryos (C-F;
line no. 23, see Table 1), and
transverse cardiac sections of E12.5 embryos (G,H; line no. 25). Note
overexpression (red arrows) in outflow tract (oft) and left ventricle (lv; D),
as well as ectopic expression (thick red arrows) of the transgene in the atria
of Tg mice (F,H); asterisks indicate lack of endogenous and transgenic
Lbh expression in the conotruncal endocardial cushions in both wt (G)
and Tg (H). at, common atrium; avc, atrioventricular canal; lb, limb bud; la,
left atrium; ra, right atrium; rv, right ventricle; so, somites; sv, sinus
venosus.
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