DEV01888 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure 1 -

  Fig. S1. Vessel enlargement is associated with increased numbers of endothelial cells and endothelial cell proliferation. (A,B) Tracheal vessels were stained with a monoclonal antibody against CD31 (green), which selectively stains endothelial cell junctions, and a nuclear stain (ToPro3 – not shown for clarity) to mark individual cells. The number of endothelial cells around the vessel circumference (asterisks) was assessed. More endothelial cells per circumference were found in tracheal vessels following treatment with ANG1^4FD. (C,D) BrdU labeling of tongue vessels in paraffin sections shows enlarged vessels and endothelial cell proliferation (arrows) following treatment with ANG1^4FD. Additional labeling with BrdU was found in the basal epithelial cells (arrowheads), but these cells were present in tissue from both control and ANG1^4FD-treated mice.