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Fig. 2. Vessel morphology in mouse tissues after treatment with angiopoietin 1.
Mouse pups were treated daily for 7 days with 200 µg of ANG14FD
protein. Arteries and veins were readily identified in wholemounts by virtue
of the differential coating of the smooth muscle cell layer (arterioles had a
regular layer of elongated smooth muscle cells wrapped circumferentially
around the vessels, whereas venules had an irregular layer of spread, branched
smooth muscle cells associated with the vessels) and by vessel morphology
(arteries were straighter and smaller in diameter than the corresponding
veins). (A,B) Whole-mount views of tracheas from P14 mice, with blood vessels
immunostained for PECAM (green) and 
-smooth muscle cell actin
(red/orange). The straight capillaries traverse the cartilaginous rings. The
venular ends of the capillaries are enlarged in the tracheas of
ANG14FD-treated mice (arrows, B) compared with PBS controls (A),
and the draining venules are also enlarged, whereas the arterioles
(arrowheads) are not enlarged. (C,D) Cross-sections of tongue from P14 mice,
with blood vessels immunostained for PECAM (green) and -smooth muscle
cell actin (red/orange). The upper epidermal surface of the tongue is at the
upper part of the image. The draining venules and the vessel loops in the
dermal papillae (arrows) in the muscosa and muscle layers are enlarged in the
tongues of ANG14FD-treated mice (D) compared with PBS controls (C).
By contrast, the feeding arterioles (arrowheads) are similar in size in both
ANG14FD-treated and control mice. (E,F) Whole-mount views of
diaphragm from P14 mice, with blood vessels immunostained for PECAM (green)
and -smooth muscle cell actin (red/orange). The straight capillaries
are in the skeletal muscle and the draining venules (arrows) are at the
boundary with the central tendon. The venules (arrows) in the central tendon
are enlarged in the diaphragms of ANG14FD-treated mice (F) compared
with PBS controls (E). By contrast, the muscle capillaries in
ANG14FD-treated mice and control mice are similar in size. (G,H)
Whole-mount views of retinas from P14 mice, with blood vessels stained with
Griffonia simplicifolia isolectin B4 (green). The capillaries
traverse between arterioles (arrowheads) and venules (arrows). The venular
networks (arrows) are enlarged in retinas of ANG14FD-treated mice
(H) compared with PBS controls (G).
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