DEV01912 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure 1 -

  Fig. S1. Methylation analyses of S100b promoter in neural precursor cultures and in control and Dnmt1^–/– CNS in vivo. (Left panel) Site-specific (–1135 CpG site) demethylation within the S100b promoter in E11 CNS neural precursor cell (NPC) cultures over a period of 4 days. (Right panel) DNA methylation analysis indicates that the S100b promoter is slightly demethylated as control CNS NPCs (con) gain competency of gliogenesis from E11 to E18 in vivo. In the nestin-cre; Dnmt1-conditional knockout mice, E18 Dnmt1^–/– mutant (mut) CNS cells become significantly demethylated in the S100b promoter.

• Supplemental Figure 2 -

  Fig. S2. CpG methylation attenuates the association of STAT1/3 to the STAT-binding element within the mouse Gfap promoter. (Left panel) EMSA assay of recombinant pSTAT1 association with unmethylated and methylated STAT binding elements within the Gfap promoter. Data demonstrate inhibition of recombinant pSTAT1 binding to the methylated probe (Me). (Right panel) The binding of STAT1/3 complex in nuclear extracts from 4-day-old cultured, 20 minute LIF-treated control E11.5 CNS cells to the methylated- (Me) or unmethylated control probes.

• Supplemental Figure 3 -

  Fig. S3. Expression of MeCP2 in E11.5 cortical NPCs. Western blot analysis demonstrating that the mouse E11.5 cortex, which is composed primarily of NPCs, expresses MeCP2 protein (left panel). (Right panel) Western blot analysis indicates that the level of MeCP2 protein in mouse E11.5 cortical NPC cultures does not change dramatically over a period of 8 days in culture.