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Fig. 1. Precocious astroglial differentiation in
Dnmt1–/– CNS in vivo. (A,B)
Immunohistochemistry studies indicate enhanced S100ß- and GFAP-positive
staining in E15.5 (A) and E18.5 (B) cervical spinal cords from littermate
control (con) and Dnmt1–/–mutant (mut) mice. Bottom
rows show enlargements of the boxed areas in the tops rows. (C,D) Western blot
analysis of GFAP (C) and S100ß (D) proteins in E12-18 spinal cords at
cervical/thoracic level. (E) GFAP immunostaining (red, arrows) of the E18.5
cortical VZ/SVZ in coronal sections (relatively caudal regions) (LV, lateral
ventricle; D/M, dorsal/medial; V/L, ventral/lateral). (F) GFAP immunostaining
of E15.5 hippocampal primordial areas. Scale bar: 157 µm. (G) Western blot
analysis of GFAP protein in E18.5 brain samples. CTX, cortex; CB, cerebellum;
TH, thalamus; STR, striatum; OB, olfactory bulb. Reblotting with an antibody
against ßIII-tubulin serves as an internal control for loading. (H)
Northern analysis of GFAP mRNA in E18.5 whole brain samples. con, control;
mut, Dnmt1–/–.
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