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Fig. 3. Enhanced activation of JAK/STAT signaling in hypomethylated NPCs. (A) Western blot analysis of total STAT1 and pSTAT1 proteins in 1 day (1 D), 4 day (4 D) and 7 day (7 D) cultured E11.5 wild-type cortical cells with transient 20 minute LIF treatment. (B) Western blot analysis of total and phosphorylated STAT1/3 protein in 4-day-old cultured control (con) and Dnmt1–/– (mut) E11.5 CNS cells with 20 minutes LIF treatment. (C) Northern blot analysis of STAT1 and STAT3 mRNA in E18.5 CNS samples. (D) Western blot analysis of gp130 receptor protein in E18 cortices. con, control; mut, Dnmt1–/–.





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