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Fig. 4. Detection of a new class of vesicle by electron microscopy. (A) Low-power
views of vesicles (arrows) in the extracellular space near spermatozoa (s) in
the spermatheca (left), in the spermathecal-uterine junction region (middle),
and in an extracellular space of the uterus formed by close packing of embryos
(e; right). Pseudopods (ps) and an apical junction between spermathecal cells
(arrowhead) are indicated. (B) Serial-section analysis of two vesicles in the
spermatheca. Inset is a magnified view. Sections are 75 nm thick. (C) The
vesicles possess two concentric lipid bilayers. Vesicles were tilted through
the indicated angles in the EM beam to visualize the individual leaflets of
the inner and outer membranes. (D) Tilting of a vesicle to visualize its
scalloped appearance. (E) No MSP vesicles are observed in the spermathecal
lumen (sl) of a fog-2(q71) female, instead the lumen is filled with
material that resembles yolk lipoprotein particles. Scale bars: in A, 500 nm;
in B, 125 nm; in C,D, 100 nm; in E, 500 nm.
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