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Fig. 6. Spermatids provide a long-acting MSP signal. (A) Time-course analysis of
MAPK activation in the wild type and spe-8(hc50) mutants. The
percentage of gonad arms with activated MAPK was measured by staining
dissected gonads at the indicated times of adulthood. (B) Time course of total
MSP levels analysed by western blots of MSP in spe-8(hc50) and the
wild type (10 animals/lane). The number of spermatids and spermatozoa were
counted at each time point. The data represents the average of three trials.
(C) Detection of MSP puncta (arrows) located near spermatids (sd) in the
proximal gonad arm of spe-8(hc50) and wild-type hermaphrodites. MSP
(red) and DNA (blue) were detected. MSP is detected in the spermatheca (sp) of
spe-8(hc50) hermaphrodites, but no spermatids are observed. (D)
Projection of a confocal 3D-data stack showing MSP puncta (arrow) distributed
widely in the proximal gonad, far from the single spe-8(hc50)
spermatid (sd) that can be seen. (E) MSP perdures in spe-8(hc50)
mutants. MSP (red) staining is observed, but spermatids are not, confirmed by
viewing the DNA (blue) signal in multiple focal planes. (F) Western blot of
MSP in C. elegans (C.e), and the Cephalobid nematodes
Acrobeloides maximus (A.m) and Zeldia punctata (Z.p). (G)
Detection of MSP puncta (arrows) in the A. maximus gonad. Only the
distal arm is shown. Scale bars: in C, 20 µm; in D, 10 µm; in E,G, 20
µm.
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