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Fig. 7. Production of MSP vesicles in the spermiogenesis-defective
spe-8(hc50) mutant. (A) Low-power view of the spermathecal-uterine
junction region. MSP vesicles (arrows) are abundant in extracellular spaces of
this region. (B) High-magnfication view of MSP vesicles located in the region
shown in A, from an adjacent section. (C) MSP vesicle in the spermathecal
lumen surrounded by cytoplasmic debris from a lysed oocyte (arrowheads). Note,
the inner ring of the MSP vesicle contains material (arrows) similar to the
oocyte cytoplasmic contents. (D-I) Protrusions (arrows) from the cell body of
spermatids located in the gonad arm. (F,I) Detection of MSP in protrusions.
Scale bars: in A, 500 nm; in B-I, 100 nm.