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Fig. 5. Optic pathway apparently unaffected by MMP inhibition. Embryos were exposed
at stage 31 to either a control solution (A,C,E,G,I,K) or 25 µM SB-3CT
(B,D,F,H,J,L). At stage 37/38, embryos were fixed. (A-D) Embryos were
processed as wholemounts for in situ hybridization for Xenopus slit
mRNA. Vibratome sections (50 µm) were cut. The ventral diencephalon at the
level of the optic chiasm is represented in A,B, and eye sections are shown in
C,D. (E-H) Immunoreactivity for the glial cell marker 3CB2. (G,H) Higher power
views of E,F, showing the optic nerves and the optic chiasm in the ventral
diencephalon. (I,J) Islet 1 immunoreactivity showing label in the ventral
region of the diencephalon adjacent to the optic chiasm, and labelling of RGCs
and cells in the inner nuclear layer in the retina. (K,L) GABA
immunoreactivity showing labelling of amacrine cells in the inner nuclear
layer, and a population of neurons in the ventral diencephalon. L, lens; nc,
notochord; ap, anterior lobe of the pituitary body; nr, neural retina; PE,
pigment epithelium; oc, optic chiasm; on, optic nerve, RGCL, RGC layer; Am,
amacrine cells; INL, inner nuclear layer; ONL, outer nuclear layer; Di,
diencephalon. D, dorsal; V, ventral. Orientation in A is for A,B,E,L, and
orientation in C is for C,D. Scale bars: in A, 50 µm for A,B; 25 µm for
C,D; in E, 50 µm for E,F,I,L; 25 µm for G,H.
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