|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 2. Triple depletion of Bmp2, Bmp4 and Bmp7 results in greater dorsalization of
the embryo than any single or double BMP knockdown. Embryos were fixed at
tailbud stage and expression domains of four markers (Otx2, Krox20,
Myod and Sizzled) were compared with control siblings
(n=15 per experimental set). (A-H) Lateral view, anterior to the
left. (A'-H') Ventral view, anterior to the top. (H'') Dorsal
view, anterior to the top. (A-D') Uninjected controls and single Bmp2,
Bmp4 and Bmp7 knockdowns. (E-G') Double BMP knockdowns (2/4, 2/7 and
4/7). Note the dorsalized phenotype with increased Otx2 expression,
radial expression of Myod in the posterior, and ventral loss of
Sizzled. (H-H'') Triple knockdown of Bmp2, Bmp4 and Bmp7
produces further dorsalization. Expression of Krox20 in rhombomere 5
was seen over the entire circumference of the embryo down to the tip of the
tail (white arrowheads).
|
