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Fig. 3. BMP knockdown induces neuronal differentiation in posterior epidermis and cooperates with Fgf8 signaling. (A) The differentiated neuronal marker N-Tubulin is expressed in the CNS of uninjected control embryos at tailbud stage (inset: dorsal view, anterior to the top). (B-D) Inhibition of endogenous Bmp4, Bmp2/Bmp4 and Bmp4/Bmp7 activities results in ectopic neuronal differentiation in the posterior ectoderm (inset: dorsal view, anterior to the top). Comparable results were obtained with Bmp2/Bmp7 and triple Bmp2/Bmp4/Bmp7 MO injections (data not shown). (E,F) Control embryos or Bmp4 MO embryos were injected at the blastula stage with 1.5 ng of recombinant mouse Fgf8 protein into the blastocoele cavity and analyzed at tadpole stage for N-Tubulin expression. Fgf8 protein injection in wild-type embryos resulted in minor ectopic neural differentiation, which greatly synergized with Bmp4 depletion.





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