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Fig. 8. Expression of Nieuwkoop, BCNE or Spemann organizer genes is not restored in
ß-Catenin/Bmp4/Bmp7-depleted embryos. (A-D) Expression of Xnr6
in the vegetal dorsal side at blastula stage 9 in the Nieuwkoop center
requires ß-Catenin, is unaffected by knockdown of Bmp4/Bmp7, and is not
re-expressed in ß-Catenin/Bmp4/Bmp7 triple knockdowns. (E-L) Expression
of the BCNE center genes Pintallavis/Hnf3ß and Chordin
at blastula stage 9 requires ß-Catenin, is not affected by the depletion
of Bmp4/Bmp7 and fails to be rescued in ß-Catenin/Bmp4/Bmp7 triple
knockdowns. (M-P) Chordin expression in the Spemann organizer at
gastrula stage 10 is eliminated by ß-Catenin depletion but is unchanged
in Bmp4/Bmp7-depleted embryos. Note that embryos shown in panels D,H,L and P
do not express any of the dorsal genes tested, yet still give rise to
hyperdorsalized embryos with large head structures. (Q) RT-PCR analysis at
stage 9. Dorsal markers are not expressed in embryos lacking ß-Catenin or
ß-Catenin/Bmp4/Bmp7. (R-V) A simplified model highlighting the
interactions taking place at blastula stage between BMP signals and the dorsal
BCNE and Nieuwkoop centers.
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