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Fig. 2. The Gata4 G2-lacZ transgene is expressed in the lateral mesoderm and septum transversum during mouse embryonic development. Whole-mount (A,B,F), transverse (E,M,O) and sagittal (J) sections of X-gal-stained G2-lacZ transgenic embryos are shown. For comparison, whole-mount (C,D,G,H), sagittal (I,K,L) and transverse (N,P) section in situ hybridization with different mesodermal markers is shown. (D,H,L) Foxf1; (G,K) Bmp4; (C,I,N) Gata4. (A,B) At 7.75 dpc and 8.25 dpc, lacZ expression directed by the Gata4 G2 enhancer is present in the lateral mesoderm (LM) and allantois (al). (E) A transverse section at midgut level at 8.0 dpc, ß-galactosidase activity is evident in both the somatic mesoderm (SM) and visceral mesoderm (VM) of the transgenic embryo. (F,J,M,O) By 9.5 dpc, lacZ expression is very robust in the mesodermal component of the septum transversum (ST) and in the visceral mesoderm surrounding the gut. Note that transgene expression is completely absent in the hepatic endoderm (HE) within the ST (F,J), and in the visceral endoderm (VE) of the gut (O). The expression directed by the Gata4 enhancer overlaps the expression of the mesodermal forkhead gene Foxf1, Bmp4 and endogenous Gata4 at all time points examined. NT, neural tube. Asterisks denote expression of the G2-lacZ transgene and endogenous Gata4 in the allantois at 8.25 dpc. Scale bars: 100 µm.

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