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Fig. 3. Expression directed by the Gata4 lateral mesoderm enhancer becomes
restricted to the mesenchyme surrounding the liver. Representative
X-gal-stained transgenic embryos (A-C) and dissected livers from transgenic
animals (E-H) are shown. (A-C) Expression directed by the Gata4
enhancer is present exclusively in the liver (L) of transgenic embryos at 11.5
dpc (A), and this expression is restricted to the mesenchyme surrounding the
liver, as observed in sagittal (B) and transverse (C) sections. The liver
mesenchyme expression directed by the enhancer is identical to the expression
of endogenous Gata4 in the liver, which was evident in transverse
sections at 11.5 dpc (D). (E-G) Expression directed by the Gata4
enhancer in the liver mesenchyme was strong at 11.5 dpc (E) but began to
diminish by 13.5 dpc (F) and 16.5 dpc (G). No transgene expression was
observed in the adult liver (H). NT, neural tube. The arrows in C and D denote
expression of G2-lacZ and endogenous Gata4 in the mesenchyme
surrounding the liver at 11.5 dpc. Scale bars: in B-D, 100 µm; in H, 1
cm.
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