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Fig. 8. The Gata4 lateral mesoderm enhancer is dependent on conserved Forkhead and GATA sites for its function in vivo. The wild-type Gata4 enhancer transgene construct G2{Delta}2 (wt; A,D,G) and transgenes containing mutations either in the Fox I site (mFox; B,E,H), or in both the gata I and gata II sites (mGATA; C,F,I), in the context of G2{Delta}2, were used to generate transgenic embryos. Representative transgenic embryos are shown at 7.75 dpc (A-C), 9.5 dpc (D-F) and 11.5 dpc (G-I). The wild-type construct directed strong expression in the lateral mesoderm (arrowhead) and allantois (al) at 7.75 dpc (A), in the septum transversum (ST) and visceral mesoderm (arrowhead) at 9.5 dpc (D), and in the mesenchyme surrounding the liver (L) at 11.5 dpc (G). Mutations in the Fox I site completely eliminated transgene expression in all of the fifteen independent transgenic embryos analyzed (B,E,H). Similarly, mutation of both GATA sites completely eliminated lacZ expression in the fourteen independent transgenic embryos analyzed (C,F,I). Arrowheads in A-C indicate lateral mesoderm; arrowheads in D-F indicate visceral mesoderm.





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