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Fig. 2. Genetic regulation of wntD expression. (A-F) The expression of
wntD and sim in wild-type (WT) embryos. Both genes have
lateral stripes of expression. Only wntD shows a lower level of
expression in ventral cells, and only sim has a characteristic stripe
in the posterior region (arrows in C and E). The embryo in E and F showed both
characters, indicating that it contained both wntD and sim
probes. Panels B, D and F are higher magnifications of the regions indicated
by the brackets in A, C and E, respectively. The double in situ hybridization
(E,F) shows that the wntD and sim patterns overlap in the
mesectoderm. (G) In embryos laid by dorsal–/–
mothers, no wntD staining was observed at any stage of embryogenesis.
(H) In embryos laid by Toll10b mothers, an expansion of
wntD expression into the dorsal side was observed. (I) Heterozygous
snail embryos had increased expression in ventral cells but the
mesectodermal expression was unchanged. (J) In the snail homozygous
background, the mesectodermal expression of wntD disappeared, and the
ventral staining became stronger. (K) In twist mutant embryos, the
wntD pattern was narrower but overall was similar to that observed in
wild-type embryos. (L) In twist snail double-mutant embryos, the
mesectodermal staining disappeared, while a weaker ventral staining remained.
(M) Sagittal view of a wild-type embryo during germ-band extension, showing
wntD expression in the neuroectoderm. (N) In embryos that were
zygotically mutant for Delta, the wntD expression in the
neuroectoderm disappeared.
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