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Fig. 4. WntD regulates Dorsal nuclear localization. All the panels show
immunofluorescence staining using an anti-Dorsal antibody. A,D,G,J and L are
side views; M and P are ventral views of whole embryos. B,E,H and K are
sagittal views, after 2D deconvolution, of the regions indicated by the
brackets in A,D,G and J, respectively. C,F and I are ventral views of
gastrulae. N and Q are higher magnification views of the posterior regions of
the embryos shown in M and P, respectively. O and R are sagittal views, after
2D deconvolution, of cellular blastoderms at the posterior region, including
the pole cells. The genotype of each embryo is shown at the bottom right-hand
corner. gd, gastrulation-defective; Toll10b is a
gain of function Toll. (A-C) In wild-type blastoderm and gastrula,
Dorsal protein is localized in the ventral nuclei. (D-F) In
gastrulation-defective mutant embryos, the Dorsal protein remains
cytoplasmic. (G-I) In many WntD-overexpression embryos, Dorsal is also
cytoplasmic. (J,K) In Toll10b embryos, the nuclear Dorsal
staining extended into the dorsal side of the embryo. (L) Essentially no
signal was detected in a Dorsal protein null embryo. (M,N) Ventral view of a
wild-type gastrula showing high levels of nuclear Dorsal around the ventral
furrow. The higher magnification in N shows that the posterior region (right
side) had less staining. (O) Sagittal view of a wild-type cellular blastoderm
at the posterior end, showing the staining of Dorsal changing from nuclear to
cytoplasmic in cells ventral to the pole cells. (P-R) Embryos derived from the
Df(3R)l26c strain, which has many genes, including wntD,
uncovered, showed increased Dorsal nuclear staining in the posterior region,
as indicated by the arrow. (R) In a cellular blastoderm before germ-band
extension, the nuclear staining of Dorsal already extents further to the
dorsal side, using pole cells as a reference.
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