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Fig. 5. Loss of WntD function leads to expansion of a Dorsal target gene. The blue
staining in all the panels is RNA in situ staining using an antisense
snail probe. The brown staining in E and F is in situ staining using
an antisense huckebein probe. (A) Sagittal view of a wild-type
blastoderm. The bracket at the posterior end indicates the retracted
expression from the pole. (B) Ventral view of a wild-type gastrula, showing
the sharp pattern of snail in the lateral and posterior regions. (C)
Sagittal view of a Df(3R)l26c blastoderm. The bracket and the arrow
indicate the expanded staining in the posterior and anterior regions,
respectively. (D) Ventral view of a Df(3R)l26c gastrula; the expanded
staining is similarly indicated by the bracket and the arrow. (E,F) Double
staining of snail and huckebein, showing their complementary
patterns in the posterior region of a wild-type embryo but overlapping pattern
in a Df(3R)l26c embryo. (G) Ventrolateral view of an embryo derived
from Df(3R)l26c strain that also contained a transgenic wntD
genomic construct. All embryos from this rescued strain showed snail
expression identical to that observed in wild-type embryos. (H) Ventral view
of another wild-type blastoderm, with a retracted posterior pattern. (I)
Sagittal view of a wild-type blastoderm previously injected with wntD
dsRNA, showing a slightly expanded posterior expression. (J) Ventral view of a
wild-type blastoderm previously injected with wntD dsRNA. The
posterior sharpening is not as obvious as in wild-type embryos injected with
buffer alone.
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