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Fig. 2. Foxa1 regulates prostate ductal morphogenesis. (A) Upper panels: urogenital
organs (lateral, left, and dorsal, right, views) dissected from P1 pups. The
bladder was removed as indicated by broken lines. Lower panels: prostate
rudiments (asterisks) and seminal vesicle (SV) were grafted as renal rescue
tissue. (B-D) Upper panels: 8-week-old rescued tissues, with indicated
genotypes, developed in the host renal capsules. SVs are circled with broken
lines. Rescued Foxa1–/– prostate (asterisk in
D) is smaller than controls upon comparison after fine dissection (lower
panels). (E-G) ß-Galactosidase staining on 8-week-old rescued prostates.
(H) Twelve-week-old tissue recombinants derived from wild-type (left) or
Foxa1–/– (right) epithelium that was
recombined with E18 rUGM. (I) The Foxa1–/–
recombinants have significantly lower weights than controls (n=3,
P<0.01). (J-K) Hematoxylin and Eosin staining of 4-week-old
rescued Foxa1–/– and
Foxa1+/+ VPs. (L,M) AR staining. (N,O) High magnification
views of regions framed in L and M (arrowheads). (P,Q) Toluidine Blue staining
on 1 µm thin section of 12-week-old rescued
Foxa1–/– and wild-type VPs. (R,S) E-cadherin
staining (red) on 12-week-old rescued Foxa1–/–
and wild-type VPs. Foxa1-null epithelial cell polarity is disrupted
(arrowheads). (T,U) Hematoxylin and Eosin staining of 12-week-old tissue
recombinants from Foxa1–/– and control
epithelium.
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