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Fig. 5. No mature luminal cells in Foxa1–/– prostate. (A-D) EM analysis on 12-week-old rescued Foxa1+/+ and Foxa1–/– VPs. Scale bars: 2 µm in A,B; 500 nm in C,D. Asterisks indicate the lumen. Wild-type cells (A,C) contain secretory materials seen in apical vesicles (arrow) and at luminal surface (arrowhead). Secretory material was absent in Foxa1–/– cells (B,D). (E) MALDI-MS protein profiles of m/z range 3000-16,000 obtained from 4-week-old rescued Foxa1+/+, Foxa1+/– and Foxa1–/– prostates. Continuous profiles were zoomed in at m/z range 16,100-26,000. Arrows indicate peaks that were absent in Foxa1–/– but present in control prostates. (F) RT-PCR for Pbsn and Sbp. Bs, basement membrane.





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