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Fig. 2. Cytokeratin in vegetal cortex of stage VI oocytes. (A) Confocal images of the vegetal cortex of stage VI oocytes, oocytes matured in vitro and eggs activated by pricking stained with anti-pancytokeratin C11 antibody conjugated with FITC. Left panel, stage VI oocytes. Central panel, oocytes matured in vitro with progesterone. Right panel, activated eggs. Control mock-injected stage VI (1) and matured (2) oocytes (arrows indicate cytokeratin foci). (3) Control oocyte after activation. (4) Stage VI oocyte injected with antisense Xlsirts ODNs (short arrow indicates yolk platelet). (5) Matured antisense Xlsirts-injected oocyte (arrows indicate cytokeratin foci). (6) Activated egg from antisense Xlsirt-injected oocyte (short arrow indicates yolk platelet). (7) Stage VI oocyte injected with antisense VegT ODN. (8) Antisense VegT ODN-injected oocyte that has matured. (9) Activated antisense VegT-injected egg showing only separated short filaments of cytokeratin (arrows). (B) Stage VI oocyte injected with antisense Xpat (1), antisense XlCaax (2) ODNs or VegT morpholino (3). Scale bars: 12 µm.





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