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Fig. 3. Anti-pancytokeratin C11 antibody disrupts cytokeratin network in stage VI
oocytes. Anti-pancytokeratin C11 antibody conjugated with FITC was injected
into the cytoplasm of stage VI oocytes. At various time points, oocytes were
fixed for 5 minutes in 1% formalin in PBS and vegetal cortexes were observed
under fluorescent microscope. (A) Up to 30 minutes from the injection of
antibody. (B) Three hours after the injection of antibody. (C)
Antibody-injected progesterone matured oocyte. (D) Antibody-injected, matured
and activated (by pricking) oocyte. (E) Antibody injected oocyte post-injected
with rescuing VegT RNA shows partial reconstitution of the cytokeratin
network. Scale bars: 12 µm.
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